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http://10.1.7.192:80/jspui/handle/123456789/11066| Title: | Evaluating the In - Vivo Anticancer Potential of Doxorubicin Nanoformulations in Rodent Models |
| Authors: | Mahato, Arpita Srivastava, Vadini Pal, Udita Kakkad, Dhara Maniar, Jalak |
| Keywords: | Biochemistry Project Report Project Report 2021 19MBT 19MBC 19MMB 19MBC003 19MBC021 19MMB006 19MMB014 |
| Issue Date: | 2021 |
| Publisher: | Institute of Science, Nirma University |
| Series/Report no.: | ;SDR00393 |
| Abstract: | Introduction This study was carried out for the evaluation of the hepatoprotective effects of doxorubicin nano formulations on carbon tetrachloride (CCl4) induced liver cancer in Wistar rat models. Hepatocellular carcinoma is the most common type of liver cancer where it initiates from the main cells called hepatocytes. HCC animal models can be categorized as follows: (i) chemically induced models, (ii) genetically engineered models, (iii) syngeneic models, (iv) xenograft models including patientderived xenograft models, and (v) humanized models. In the present study, it is shown CCl4 causes severe damage in liver resulting into cancer development in future. It also affects kidneys, lungs, and brain. Gut microflora also plays a vital role in HCC. Alteration in gut microflora and gut permeability play a role in several types of CLD and promotes to disease progression and leads to the development of HCC (Hepatocellular carcinoma). Different treatment options are available for liver cancers such as- cytotoxic chemotherapy, Immunotherapy, surgical resection, liver transplantation and ablative therapies. Here, it is tried to check anticancer potentials of nano formulations such as curcumin, cyclodextrins, dextran and α-hydroxy acids and CNT which enhance the properties of traditional drugs and are specific towards the targets or the targeted delivery sites. Hypothesis To check the efficacy of various nano formulations along with standard anti-cancer drugDoxorubicin which is used in the treatment of liver cancer for the remission of hepatoma and to monitor the gut microflora alterations which can promote HCC progression. Objectives Our aim was to investigate the efficacy of certain nano formulations and to ascertain the role of gut microflora and its alteration in rodent models. Materials and Methods Male Wistar rats were taken as animal models. Induction of liver cancer was done using non genotoxic carcinogen CCL4. After checking the induction, reversal treatment with nano formulations was provided. At the end of the study, animals were sacrificed and their tissues, blood and fecal samples were collected and stored for further analysis. Statistical analysis was performed to determine the significance level of the data. Results and Discussion In CCl4 induced animal models it was found that there was significant increase in the levels of Cholesterol, Triglycerides, Total protein, and the concentrations of SGPT and SGOT were relatively high. So, overall, these results clearly indicate the induction of cancer due to CCl4 when the data was compared with DEN+AAF induced models. And further, if the reversal treatment with different nano formulations having anti-cancer properties and standard chemotherapeutic drug Doxorubicin would be provided then, remission in HCC could be observed. Also, restoration of the gut flora can be analyzed. Future scope of the study Liver cancer is treatable but sometimes, at some stage it becomes difficult to cure it completely. This study would provide information on the efficacy of certain test compounds – nano formulations which are target – specific so it minimizes side effects/adverse effects so may help to cure liver cancer. Such approaches can be useful to improve cancer related treatment strategies with fewer side effects |
| Description: | SDR00393 |
| URI: | http://10.1.7.192:80/jspui/handle/123456789/11066 |
| Appears in Collections: | Dissertation, BC |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| SDR00393.pdf | SDR00393 | 4.09 MB | Adobe PDF |  View/Open |
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