Effect of Polyherbal Oil on WNT Signalling Mediated Regeneration During Diabetic Wound Healing

Abstract

Any injury to the body is repaired as soon as possible through natural physiological processes known as 'wound healing.' The fourth phase, Remodelling is an important phase that involves the formation of new epithelium and scar tissue during wound healing. Wnt 10b plays major role in tissue remodelling. Wnt 10b binds to FZD-6 on the cell surface, which activates Wnt signalling pathway, which contributes to tissue remodelling in wound healing process. Polyherbal oil is an ayurvedic medicine that helps to fasten wound healing process. Constituents of polyherbal oil have been found to enhance wound healing by influencing the Remodelling phase. Thus, in the present study, Wnt 10b expression has been evaluated during wound healing process with respect to Polyherbal oil treatment during diabetic condition. In the in-vivo study, wound was induced in both, control as well as in diabetic wistar rats, and the treatment groups were topically treated with Polyherbal oil twice a day for the duration of the experiment. Every day, pictures of the wound were taken to analyse the rate of wound closure, which was found to be faster in control and even diabetic groups after Polyherbal oil treatment compared to untreated groups. HE was used to evaluate histological changes and skin regeneration after treatment of wounds. Histology revealed that Polyherbal oil treatment enhances wound healing by improving epidermis regeneration via re-epithelization and keratin synthesis, adipocyte proliferation, fibroblast proliferation and migration, and muscle regeneration in non-diabetic control rats. As a result, wound closure occurs at a faster rate. This project also aimed to analyse and screen potential drug-like molecules (phytochemicals) found in plant sources that can interact with Wnt 10b. This was accomplished through ADMET, molecular docking, and pharmacophore generation studies, and with the use of various in silico pharmacokinetics tools.