In-Vitro Drug Release of Dolutegravir Sodium, Lamivudine and Tenofovir Fumarate Tablet by RP-HPLC Analysis from its Combined Dosage Form and Microwave Assisted Hydrolytic Degradation of Fluconazole and its Comparison with Conventional Heating Method using RP-HPLC

Abstract

Dolutegravir Sodium, Lamivudine Tenofovir Fumarate are orally administrated, Dolutegravir Sodium belongs to the class of HIV Integrase Inhibitors whereas Lamivudine Tenofovir belong to NRTIs category used in the treatment of HIV A simple, specific, linear, accurate, robust, sensitive and precise high performance liquid chromatography method was developed for Dolutegravir Sodium, Lamivudine Tenofovir Fumarate The column used Intersil ODS 3 150 mm* 4 6 mm, column packed with 5 μm particles, mobile phase was a mixture of buffer solution, and water, methanol OPA in the ratio of 10 90 0 1 v/v/v was pumped at a flow rate of 1 ml/min Detector wavelength was monitored at 260 nm for Dolutegravir and Tenofovir 285 nm for Lamivudine, and the injection volume was 15 μL and run time was kept 12 min The developed method was validated in terms of linearity, range, accuracy, precision, repeatability and specificity, robustness as per ICHQ 2 (R 1 The method had more linearity in the concentration ranges of 0 6 to 75 μg/mL) having with regression coefficients of 1 000 The recovery ratios were in the range of 95 105 The precision was evaluated by studying intermediate precision and repeatability precision was performed in a similar way another day The RSD values for method precision were 0 07 and intermediate precision were 0 4 respectively