Investigation of Neuroprotective Effect of Ethanolic Extract of Nardostachys Jatamansi in Aluminium Chloride Induced Pathophysiology Associated with Alzheimer's Disease

Abstract

1.1 Background Dementia, mental confusion, repeated speech, and social disengagement are symptoms of the neurodegenerative disease Alzheimer's. Reduced acetylcholine levels, oxidative stress, the formation of amyloid plaques, tau protein hyper phosphorylation that results in neurofibrillary tangles, etc. are some of the reported pathogenic pathways for AD. NMDA receptor antagonists and acetylcholinesterase inhibitors have both been FDA-approved to date, however they only provide symptomatic relief and are unable to stop the progression of disease. Nardostachys Jatamansi is flowering plant of the valerian family that grows in the Himalayas and reported for anti–oxidant, anti-inflammatory, immunomodulatory and neuroprotective activities. 1.2 Aim and Objective The aim of the study is to investigate the neuroprotective effects of ethanolic extract of Nardostachys Jatamansi and its mechanism of action against Aluminium chloride induced Alzheimer’s disease model in rats. 1.3 Methods Procedure: Rats will be used for the study, divided in 05 groups. The groups are healthy control, aluminium chloride treated disease group and treatment groups. Control will be treated with normal vehicle. Aluminum chloride disease groups will be treated with AlCl3 for 28 days. The treatment groups will be treated with the Nardostachys Jatamansi along with AlCl3 with different doses of NJ. Behavioural studies will be assessed by using maze test i.e, Morris water maze test and elevated plus maize method. Lastly animals will be sacrificed and brain will be isolated for biochemical and histopathological studies. Histological examination of brain tissue should be performed to determine presence of amyloid beta plaques or tau tangles. 1.4 Results In Neurobehavioural parameters, will be decreased escape latency time to reach the platform in Morris water maze test and elevated plus maize method in animals treated with Nardostachys Jatamansi. 1.5 Conclusion Collective data of neurobehavioral parameters, biochemical estimations and histopathological analysis indicates significant improvement in cognitive functions will be seen,reduction in Tau tangles and neuro inflammation, in the Nardostachys Jatamansi treatment groups in a dose dependent.