Study Effect of TurmiZn on the Cognitive Impairment Due to Gut Brain Axis: A Novel Approach for Treatment of Gut Brain Disorder

Abstract

Background: Gut brain axis is a bidirectional communication between gut and brain and it is associated with many diseases such as depression, anxiety, cognitive impairment which leads to Alzheimer’s disease, Parkinson’s disease and many more. In case of leaky gut, pathogens and toxins travel across the intestinal barrier and circulate in peripheral system. In case of leaky gut toxins or inflammatory mediators crosses BBB and travel to the brain and cause neuroinflammation. Currently there is no FDA approved drug for the gut brain axis dysfunction. TurmiZn is a novel compound with a combination of Zn2+ and Curcumin. TurmiZn have anti-inflammatory and anti-oxidant properties and it also has protective effect on Gut. Objectives: The objectives of this study are to evaluate the neuroprotective as well as pharmacological effect of TurmiZn against cognitive impairment. Materials and Methods: The study was carried out to evaluate the effect of TurmiZn. For the study, animals were divided into 7 groups Normal Control, Disease Control, Control treated with test drug (TurmiZn 50mg/kg p.o.), Reference standard group (Curcumin 50mg/kg p.o.) and treatment group (TurmiZn 10mg/kg, 25 mg/kg, 50 mg/kg p.o.) for 21 days. For inducing the disease heat stress was used. In heat stress method animals were transferred from their home cage to the new cage and animals are placed in a pre-heated climatic chamber for 45o C and 15 min with humidity of 60+10oC. Heat stress was given for 21 days and various parameters were evaluated. Animals were sacrificed on 28th day, blood was collected for evaluation of Haematology parameters. Brain was isolated and used for Oxidative parameters, inflammatory parameters, Neurotransmitter parameters and histopathology study. Results: Pre-treatment with TurmiZn reduces the colon permeability and the brain function was found unaltered in all the groups which were assessed by behavioural tests, AchE activity, 5-HT level estimation and histopathology. TurmiZn showed positive results in the colon but no effects were observed in brain. TurmiZn reduces pro-inflammatory cytokines and also reduces the glutathione levels, increases SOD activity. It shows anti-inflammatory and anti-oxidant properties in the colon, however the brain glutathione level and SOD activity was found unaltered in all the groups. Conclusion: Chronic exposure to heat stress induced altered colon permeability however brain function was unaltered. TurmiZn pre-treatment of 25mg/kg and 50 mg/kg shows protective effect in colon against heat stress induced model. TurmiZn pre-treatment reduces the oxidative stress, shows anti-inflammatory effects in colon.