Green Analytical Method Development and Validation of Levobupivacaine Hydrochoride by RP-HPLC

Abstract

Based on Chromatographic separation, two eco-friendly techniques were developed for Levobupivacaine Hydrochloride. Levobupivacaine Hydrochloride (LBH) is an Anesthetic drug that is used as a local anesthetic. The Green Analytical Chemistry approach was incorporated for the development of analytical methods. The first method was High- Performance Thin Layer Chromatography (HPTLC). NP-HPTLC methods were green and sustainable for Levobupivacaine Hydrochloride and it was detected at wavelength 210 nm. The green mobile phase optimized was N-propanol: Ethyl Acetate (60:40% v/v) G-NP- HPTLC. Here, Rf values were 0.56. The linearity range was 500-2500 ng/band in normal phases. The second method was High Performance Liquid Chromatography (RP-HPLC) with UV as a detector for quantification of a drug at wavelength 204 nm over a linearity range of 10-70 ppm with for Levobupivacaine Hydrochloride. The green mobile phase optimized was Iso-propanol: Buffer (phosphate buffer pH:5) (30:70% v/v) for G-RP- HPLC. Both techniques were evaluated for greenness assessment using AGREE tool and the green value NP HPTLC were found to be 0.75 and for RP-HPLC it was 0.73 suggesting an excellent greenness profile. Both methods are simple, accurate, precise, specific, and green for the estimation of Levobupivacaine Hydrochloride in pharmaceutical API forms.