A Study on the In Vitro Antioxidative Property of Some Plant Extracts and the In Vitro Effect of Stevia Rebudiana Extract against Lindane Liver Toxicity

Abstract

Antioxidants are the substances able to prevent or inhibit oxidation processes in human body as well as in food products. The important roles of reactive oxygen species in diseases related to aging and the necessity and benefits of antioxidative nutraceuticals in the prevention of diseases and promotion of healthy aging have been extensively reported in recent years. The natural antioxidants are a stable part of nutrition as they occur in almost all edible plant products. In current project, plants (Prosopis cineraria, Rhus myserensis Cordia dichotoma) from arid zone of INDIA and Stevia rebaudiana were investigated for their antioxidative activity in RBC model system using parameter like SOD, MDA, reduced glutathione. The synthetic antioxidant BHT (butylated hydroxyl toluene) was used as standard and oxidative stress induced using H2O2. These extract also used in the study for their prevention effect against oxidative damage using pBR322 plasmid DNA treated with H2O2 for inducing oxidative stress. These extract prepared using peels/fruits/seeds/leaves of the plant by cold extraction in solvent like water, methanol, and acetone. Out of all ,extract acetone extract of Stevia rebaudiana leaves exhibit best antioxidant activity and it was further investigated in vivo in wistar rats for its probable curative effect against lindane induced liver toxicity using parameters like AFP, SGPT, SGOT, GGT, ALP, ALBUMIN. Positive results have been obtained after 15 days of treatment and the treatment will be continued for about 1 month.