Evaluation of Antihyperlipidemic Activity of Various Combinations of Safflower Oil and Rice Bran Oil on Triton WR-1339 Induced Hyperlipidemia in Rats

Abstract

Aim and objective: The present study was undertaken to evaluate the antihyperlipidemic activity of various combinations of Safflower oil (SO) and Rice bran oil (RBO) on triton WR 1339 induced hyperlipidemia in rats. Materials and Methods: Wistar rats of either sex were utilized for the study. SO, RBO, Combinations of SO and RBO and atorvastatin were administered for 30 days. Hyperlipidemia was developed by single intraperitoneal injection of triton WR 1339 on 31st day. Rats were divided in 8 groups , Normal control ( NC), triton WR 1339 control , triton WR 1339 + SO ( 7 ml/kg, Orally), triton WR 1339 + RBO (7 ml/kg, Orally) , triton WR 1339 + [SO+RBO] ( 2:8,7 ml/kg, Orally), triton WR 1339 + [SO+RBO] (3:7, 7ml/kg, Orally), triton WR 1339 + [SO+RBO] (5:5 ,7 ml/kg, Orally) and triton WR 1339 + atorvastatin ( 2 mg/kg, Orally). After 24 hours of triton injection, the blood was collected from the retro- orbital plexus of the rat for the estimation of lipid profile and animals were sacrificed. The liver was isolated and homogenated for estimation of oxidative stress parameters. Results: The administration of triton WR -1339 showed significant increase in the level of the Total cholesterol ( TC) , Triglyceride ( TG) , Low- Density lipoprotein (LDL),Very low density lipoprotein (VLDL), Atherosclerotic index (A.I) and Malondialdehyde ( MDA) and showed a significant decrease in the level of High Density lipoprotein ( HDL) , Ratio of HDL/TC and Reduced glutathione (GSH) Pretreatment with SO, RBO, their combinations and atorvastatin showed a significant reduction in the TC, TG, LDL, VLDL, A.I , MDA except SO which showed nonsignificant reduction in the TG and VLDL level as compared to triton WR 1339 control group. These all treatments also showed significant increase in the HDL, HDL / TC and GSH level except SO which showed nonsignificant increase in HDL, HDL/TC and GSH level as compared to triton WR 1339 control group. Administration of combination of SO and RBO in 2:8, 3:7 and 5:5 proportion produced a significant decrease in the TC, TG and VLDL as compared to SO treated group. It produced a significant decrease in TC and LDL as compared to RBO treated group. Administration of combination of SO and RBO in 2:8, 3:7, 5:5 proportion produced significant increase in HDL / TC as compared to SO treated group. Administration of combination of SO and RBO in 5:5 proportion produced a significant increase in GSH level as compared to SO treated group. Conclusion : In the present study, maximum depression in Total cholesterol was seen in [SO + RBO] (5:5) proportion, maximum depression in Triglyceride and VLDL and maximum increase in HDL and HDL/TC was seen in [SO + RBO] (3:7) proportion, maximum depression in LDL and Atherosclerotic (A.I) was seen in [SO + RBO] (2:8) proportion. Maximum depression in Malondialdehyde (MDA) was seen in [SO + RBO] (2:8) proportion and maximum increase in Reduced glutathione ( GSH ) was seen in [SO + RBO] ( 5:5 ) proportion. So, our study suggests that SO and RBO in 3:7 proportion is the optimum combination as it shows the maximum depression in TG and VLDL level and it shows the maximum increase in HDL and HDL/TC which may be beneficial in treatment of hyperlipidemia. However further study is required to confirm its mechanisms for the higher effect as compared to other combinations.