Synthesis and In-Vitro Biological Activity of anti Cancer Moleceules

Abstract

The primary functions of cancer chemotherapeutic agents are not only to inhibit the growth or kill the cancer cells, but conventional cancer chemotherapy is highly inadequate as a result of the lack of selectivity between cancer cells and normal cells. This calls for novel cancer therapies for selectively targeting cancers without toxicity to normal tissues. The discovery of novel anticancer agents will hopefully provide the desired degree of selectivity for cancer cells. These novel targets include genes involved in malignant transformation, cancer progression and metastasis. In addition to the identification of many novel anticancer targets, molecular biology methods have facilitated the investigation of the potential of these targets for drug discovery. Extensive literature review revealed that compounds studied for anti proliferative activity with quinoline, pirazolopyrimidine, benzthiazole, piperazine moieties shows potent activity. Hence compound 1 containing benzthiazole, pirazolopyrimidine moieties with quinoline ring and compound 2 containing morpholine, phenol moieties with pyridine, furan and piperazine fused ring system were designed and synthesized. Structural elucidation of the synthesized compounds was done by spectral analysis. Anti proliferative activity of compounds were evaluated in vitro on U937 (human leukemic monocyte lymphoma cell line), ARH77 (Human plasma cell leukemia) and RPMI 8226 (Human myeloma cell line) by MTT assay method and Doxorubicin taking as a reference standard (1uM). Both compounds showed good anti proliferative activity against all cancerous cell lines on 48 h observation bases.