A Batch Decolorization and Kinetic Study of Reactive Azo Dye RO-13 by Novel Bacterial Strain Alcaligenes faecalis PMS-1

Abstract

An Alcaligenes faecalis strain (PMS-1) with high activity for decolorization of Reactive Orange13 (RO-13) was isolated from dye contaminated industrial site. Effects of operation parameters (initial dye concentration, temperature, pH, and salinity) on decolorization of the azo dye by PMS-1 were systematically investigated. In our experiment, an improved decolonization rate of 24.75 mg l-1 h-1 for 400 mg l-1 RO-13 dye was observed which is approximately 38.13 times higher than the reported value. The kinetic characteristics of dye decolorization by the strain PMS-1 were determined quantitatively using the monoazo dye, RO-13. The activation energy (Ea) of decolorization reaction and frequency factor (A0) were determined using Arrhenius equation. A dye decolorization kinetic study was carried out by three different approaches namely Michaelis – Menten, Lineweaver-Burk and Eadie-Hofstee. The maximum substrate consumption rate (Vmax) and decolorization rate constant (Km) were determined.