Reversed-Phase high performance liquid chromatographic (RP_HPLC) method for determination of tacrolimus in bulk and pharmaceutical formulation

Abstract

An accurate, simple, and reproducible liquid chromatographic method was developed and validated for the determination of Tacrolimus in capsules. The analysis was performed at ambient temperature on a reversed-phase C18 column with UV detection at 213 nm. The mobile phase was acetonirile (100 %) used at a constant flow rate of 0.9 ml/min. The method was validated in terms of linearity, precision, accuracy, and specificity .The response was linear in the range of 5-250 μg/ml (r2= 0.9978). The relative standard deviations for intra- and inter-day precision studies were found to be less than 2% with 98.90 to 101.12% accuracy, respectively.