Please use this identifier to cite or link to this item: http://10.1.7.192:80/jspui/handle/123456789/3331
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dc.contributor.authorPatel, Kartik-
dc.date.accessioned2012-06-05T08:46:53Z-
dc.date.available2012-06-05T08:46:53Z-
dc.date.issued2012-
dc.identifier.urihttp://10.1.7.181:1900/jspui/123456789/3331-
dc.description.abstractA rapid, sensitive and reliable method was developed to quantitate tolterodine in human plasma using liquid chromatography–electrospray tandem mass spectrometry. The assay was based on liquid–liquid extraction of the compounds from plasma with dichloromethane: isopropyl alcohol: n-hexane (40:5:55,v/v/v) and hydrophilic interaction chromatography performed on a symmetry 15cm, 150×4.6mm, 5μ column. The mobile phase consisted of acetonitrile-10 mM ammonium formate pH 5.0 (65:35, v/v). Quantification was through positive-ion mode and selected reaction monitoring at m/z 326.2 → 147.2 for tolterodine and 332.2 → 153.2 for the internal standard tolterodine D6, respectively. The lower limit of quantitation was 25 pg/ml using 0.5 ml of plasma for the parent drug and linearity was observed up to 10.00-0.025ng/ml. Within-day and between-day precision expressed by relative standard deviation was less than 11% and inaccuracy did not exceed 7% at all levels. The assay was applied to the analysis of samples from a pharmacokinetic study.en_US
dc.publisherInstitute of Pharmacy, Nirma University, A'baden_US
dc.relation.ispartofseriesPDR00187en_US
dc.subjectDissertation Reporten_US
dc.subjectPharmaceutical Analysisen_US
dc.subject10MPHen_US
dc.subject10MPH305en_US
dc.subjectPDR00187en_US
dc.titleEstimation of Tolterodine using K3-EDTA in Human Plasma by HPLC/MS/MSen_US
dc.typeDissertationen_US
Appears in Collections:M.Pharm. Research Reports, Pharmaceutical Analysis

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