Screening the organism Virgibacillus marismortui for Protease production

Abstract

We have carried out isolation of extracellular industrially important enzymes Protease from haloalkaliphilic soil organism Virgibacillus marismortui which was isolated from saline soil of Khambhat. It was observed that Virgibacillus marismortui secrete proteases which was confirmed by obtaining zone of hydrolysis on milk agar plates. Further protease activity was confirmed by zymography. We have tried to grow these organisms in different salt concentrations like 15% and 25% NaCl and determined effect of salt concentration on growth and enzyme production. This organism gives higher protease in high salt condition.