Quantitative Estimation of Amlodipine Besylate and Nebivolol Hydrochloride by Chemometrics using UV-Vis Spectrophotometry in their Combined Dosage Form

Abstract

Application of statistical and mathematical methods to solve chemical problems for maximal collection of useful information was known as “chemometrics”. Chemomatrics models, the information in the data can be extracted in an optimal way which allows us to make the best interpretation, decisions and optimization.Quantification was based on direct proportionality of concentration of analyte and absorbance in the mixture.The combination of UV-VIS spectrophotometer with chemometrics was used for the quantification of Amlodipine besylate(AMLO) and Nebivolol Hydrochloride (NEBI) used as antihypertensive and beta blocker respectively.A Partial least square (PLS) model and multivariate calibration was developed by using different concentration of both the drugs and absorbance was measured by selecting the wavelength in the range of 240-380nm. The overall % Recovery were found 99.54±1.3(PLS) and 99.31±1.39(PCR )for AMLO , 98.027±1.37(PLS) and 98.38±1.26(PCR )for NEBI respectively.The chromatographic separation was achieved on reverse-phase C8 analytical column with mobile phase consisiting of mixture of 20mM ammonium phosphate buffer(pH=2.5),acetonitrile in the ratio of (50:50) and UV detection at 291nm. A comparison of the obtained results from PLS and PCR were also performed with those obtained from reported method. The obtained t-values indicating no significant differences between the results of the proposed and reported methods. Results showed that UV-VIS spectrophotometry combined with multivariate calibration has significant potential in quantitative analysis of Amlodipine besylate and Nebivlol.