Effect of Edible oils on Myelin formation through MAL Gene Expression in Streptozotocin induced Diabetic Rats

Abstract

Neuropathy is the most common and debilitating complication of diabetes. Diabetic neuropathy encompasses a variety of forms whose impact ranges from discomfort to death. Hyperglycemia induces oxidative stress in diabetic neurons and results in activation of multiple biochemical pathways. These activated pathways are a major source of damage and are potential therapeutic targets in diabetic neuropathy. In the present study the effect of diabetes and therapeutic effect of edible oils (Coconut oil and sunflower oil) on lipid profile of streptozotocin induced diabetic rats and; enzyme assay of urea cycle enzymes such as ornithine transcarbamoylase (OTC) and carbamoyl phosphate synthase I (CPS I) were studied. A significant increase was observed in the blood glucose, food intake and water consumption, where as significant decrease in the body weight was observed in D, D+Co and D+So groups of rats compared to C. The enzyme activity of OTC and CPS1 was checked at different substrate concentrations in liver. A significant decrease in enzyme activity was observed in liver of D group compared to C. The lipid profile of experimental groups of rats has also been studied which includes triglycerides level, HDL, VLDL, cholesterol/HDL ratio. In lipid profile a significance increase in triglyceride and VLDL has been observed in D+Co group. Neuro-behavioral analysis was done to check motor activity, memory and cognition in experimental groups of rats. Impairment in motor activity was observed in D as compare to C. Memory deficit in D and D+Co was observed in Modified Karl Lashley’s maze test as compare to C.