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Please use this identifier to cite or link to this item: http://10.1.7.192:80/jspui/handle/123456789/4681
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dc.contributor.authorUpadhyay, Dhwani-
dc.contributor.authorRank, Jalpa-
dc.date.accessioned2014-07-16T08:15:02Z-
dc.date.available2014-07-16T08:15:02Z-
dc.date.issued2014-04-
dc.identifier.urihttp://hdl.handle.net/123456789/4681-
dc.description.abstractBlack mustard and Flaxseed were purchased from marked and germinated. Screening for amylase in black mustard was done by starch agar plate assay, starch – iodine tube assay and zymography. Presence of Lipase in flaxseed was confirmed after getting positive results on olive oil agar, tributyrin agar plates and zymography. Quantitative analysis of amylase was performed by DNSA method. Various types of chromatography was done ( Ion- Exchange chromatography with DEAE sephadex A-50 and Q sepharose, Gel filtration chromatography with sephadex G -75 and gel filtration high performance liquid chromatography) to purify the proteins.en_US
dc.language.isoenen_US
dc.publisherInstitute of Scienceen_US
dc.subjectBiotechnologyen_US
dc.subjectProject Reporten_US
dc.subjectBiotechnology Project Report 2014en_US
dc.subject12MBTen_US
dc.subject12MBT008en_US
dc.subject12MBT021en_US
dc.titleScreening, Isolation and Purification of Amylase from Germinating Seeds of Brassica Nigra and lipase from germinating seeds of Linum Usitastissimumen_US
dc.typeDissertationen_US
Appears in Collections:Dissertation, BT

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