First Order UV Spectrophotometric and RP-LPLC Method for Determation of Pesticides Residue in Vegetables from North Gujarat Region

Abstract

This project work describes the validated UV spectrophotometric and Reverse phase highperformance chromatography (RP-HPLC) methods for simultaneous estimation of chlorpyrifos and prophenofos in vegetables by solid phase extraction and liquid-liquid extraction. The RP-HPLC separation was achieved on a Hibar C18 column (250*4.6mm, 5μg) using mobile phase of acetonitrile:water, (90:10,v/v) as the mobile phase at flow rate 1.0ml/min. The quantification was achieved with UV detection at 219nm. UV spectrophotometric method includes first order derivative spectrophotometry method that involves the measerment of the absorbance at 277nm for chlorpyrifos and 289nm for prophenofos. Both of the methods were validated as per ICH guidelines. The comparison of liquid-liquid extraction and solid phase extraction process was also carried out for vegetable samples. The proposed methods for chlorpyrifos and prophenofos were found to be simple, precise and accurate. These methods are applicable for simultaneous estimation of chlorpyrifos and prophenofos in vegetable samples.