Effect of Hyperglycemia on Nogo Receptor Mediated Demyelination and Ornithine Transcarbamylase in Streptozotocin Induced Diabetic Rats

Abstract

Neuropathy is the most common and debilitating complication of diabetes. Hyperglycemia induces oxidative stress in diabetic neurons and results in activation of multiple biochemical pathways. A significant increase was observed in the blood glucose, food consumption and water consumption in D group of rats as compare to control where as significant decrease in the body weight was observed in D compared to C. Enzyme assay of urea cycle enzymes such as ornithine transcarbamoylase (OTC) were studied. The enzyme activity of OTC was estimated at different substrate concentrations in liver and Kidney. A significant decrease in enzyme activity was observed in liver and kidney D as compared to C. Good quality RNA was isolated from cerebral cortex which was used for the cDNA preparation. Nogo receptor specific primer was designed to study its expression. There was no significant difference was obtained for Nogo receptor expression in experimental group of rats.