Development and Validation of Zero and First Order Derivative Spectrophotometric Methods For Determination of Oxcarbazepine In Pharmaceutical Dosage Forms

Abstract

Oxcarbazepine is an antiepileptic drug. It is a 10-keto analogue of carbamazepine with a similar therapeutic profile, but with less adverse effects and less clinical relevant pharmacokinetic drug interactions. This paper describes zero and first order derivative spectrophotometric methods for determination of oxcarbazepine in pharmaceutical preparations. The solutions of the reference drug and pharmaceutical samples were prepared in methanol. Absorbance of oxcarbazepine was measured at 304.5 nm for zero order and at 266.5 nm for first order derivative spectrophotometric methods. Five brands were analysed and showed good results by both the methods. The linearity ranges were found to be 20-80 μg/ml and 10-100 μg/ml for zero and first order derivative spectrophotometric method respectively. The percentage recovery values of oxcarbazepine for both the methods were found between 98.57-101.02%. The precision (intraday, interday and repeatability) of the methods were found to be within limits. The methods developed in this study are accurate, sensitive, precise and reproducible and can be directly and easily applied to the pharmaceutical preparations