Development and Validation of HPTLC Method for The Estimation of Atomoxetine Hydrochloride in Tablet Dosage Form

Abstract

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of atomoxetine Hydrochloride in tablet dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of acetonitrile: methanol: toluene (2:4:1 v/v/v). The detection of spots was carried out at 215 nm. The method was validated in terms of linearity, accuracy, precision, specificity and system suitability. The calibration curve was found to be linear between 100 to 350 ng/spot for atomoxetine hydrochloride. Average percentage recoveries obtained was 101.83 %. The limit of detection and the limit of quantification were found to be 40 and 100 ng/spot, respectively. The proposed method is useful in the quality control of bulk manufacturing and pharmaceutical dosage forms.