Preparation and Evaluation of Resveratrol Micro Emulsion based Gel for Acne Treatment

Abstract

The aim of this study was to develop and characterize resveratrol loaded microemulsion based hydrogel for topical delivery and to enhance the solubility and penetration of the resveratrol. Using UV spectroscopy, the solubility of resveratrol in oil and surfactant was determined. The ratio and effects of the oil, surfactant, and co-surfactant on forming a transparent and stable microemulsion were investigated using pseudo- ternary phase diagrams. By using pseudoternary diagram triacetin as oil phase, tween 80 as a surfactant and PEG 400 was selected. Simplex lattice mixture design was adopted to optimize the oil content(X1), surfactant and co-surfactant content(X2), water content (X3). Globule size and % drug release was evaluated for the design batches. The optimal formulation was composed of 8% triacetin (oil), 74% tween 80 and PEG 400(Smix) and 19% water (aq.phase) and 1% resvveartrol. The optimized globule size and % drug release was found to be 22.36 nm and 80.2%. Further optimized microemulsion was incorporated into the gel formulation using carbopol 940 as gelling agent without affecting aits characteristics to increase the viscosity of the microemulsion and thereby improve the topical applicability of the formulation. In-vitro drug release of hydrogel was compared with the marketed formulation and drug release shown was 85.6% and 61.8% respectively. Thus, microemulgel can be a better alternative for the treatment of acne as compared to conventional formulations.