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Title: | Comparision and Evaluation of Flavonoid Content and Antioxidant Activity of Slected Medicinally Importance Bamboo Growing in India |
Authors: | Patel, Kuntal J. |
Keywords: | Dissertation Report Pharmaceutical Analysis 14MPH 14MPH304 PDR00382 |
Issue Date: | 2016 |
Publisher: | Institute of Pharmacy, Nirma University, A'bad |
Series/Report no.: | PDR00382; |
Abstract: | India is the second largest reserve of bamboo species after china. India about 136 bamboo species are available , among them Bambusa arundinacea , Bambusa vulgaris , Dendrocalamus strictus are indigenous species of Gujarat , declared by National bamboo mission , under the Ministry of agriculture ,government of India. Bamboo species are reported to have antioxidant activity because of its flavonoids , which can be a good source for treating various free radical mediated diseases. In the present study , comparative evaluation of antioxidant potential for selected bamboo species namely Bambusa arundinacea , Bambusa vulgaris , Dendrocalamus strictus, Phyllostachys parvifolia was carried out. Phytochemical screening of Bambusa vulgaris and Dendrocalamus strictus showed the presence of alkaloid, tannin, phenolic , flavonoids as active secondary metabolites. Quality control parameters showed , presence of more water extractable compounds. Optimization of flavonoid rich extracts shows maximum total flavonoid content and %yield in 30%ethanol leaves extracts.Antioxidant potential of leaf extract was evaluated using DPPH , Hydrogen peroxide , superoxide , nitric oxide, reducing power scavenging activites. The DPPH radical scavenging activity shows the IC50 value 89.3μg/ml , 95.51μg/ml ,142.2μg/ml,126.4μg/ml respectively as compared to L-ascorbic acid. Bamboo leaves extracts can be a good hydrogen peroxide and nitric oxide scavengers. The isolation of phytoconstituents from acid hydrolysis leaf extract was carried out by column chromatography using graded mixture of hexane:ethylacetate, chloroform:ethyl acetate, chloroform:methanol. One compound was isolated from chloroform methanol fractions(25:25,20:30)at 0.44Rf , which can be further characterized using Mass and NMR spectroscopy. |
URI: | http://hdl.handle.net/123456789/6528 |
Appears in Collections: | M.Pharm. Research Reports, Pharmaceutical Analysis |
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PDR00382.pdf | PDR00382 | 5.61 MB | Adobe PDF | ![]() View/Open |
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