Investigation into the Association of Biochemical Parameters and Gene Polymorphism with Obesityy in Patient Undergoing Bariatric Surgery

Abstract

Obesity is growing worldwide and become a major issue in the society. It has doubled since 1980. According to world health organization, people suffering from overweight in 2014 were 1.9 billion and amongst them 600 million people are obese. Obesity can be prevented by adapting certain lifestyle modification, exercise, pharmacological treatment and surgical options. Bariatric surgery is carried out on stomach and/or intestine to facilitate the weight loss. This surgery is suitable for those whose BMI is 25 or more. There are mainly 3 types of surgeries: Restrictive, Malabsorptive and combination of both. Bariatric surgery has been proven to treat complications related to obesity like type II diabetes, hypertension, PCOS, arthritis, sleep apnoea etc. According to certain studies many complications can be treated by bariatric surgery. There are certain biochemical parameters which gets changed after bariatric surgery like cardiovascular parameters, renal parameters, diabetic parameters, Thyroid parameters etc. With reference to certain study weight loss after bariatric surgery is related to genetic polymorphism. Certain genes are identified like GHSR and GLP-1. These genes are associated with weight loss after bariatric surgery. There are two main objectives in the study first is related to changes in biochemical changes after bariatric surgery and the second objective is change in the polymorphism of GHSR gene. There are very few studies carried out in India with respect to bariatric surgery and biochemical changes after bariatric surgery. Study Methodology: A. Retrospective study included diabetic and non-diabetic patients registered at Asian Bariatric Pvt. Ltd. From the available data, patients were selected according to inclusion and exclusion criteria. Medical records of 107 patients were obtained from their respective case files. The study was purely retrospective and as such only analysis of available data was carried out. The study does not involve any interaction with the subjects, nor did it require any intervention in the management of patients. B. In the second, prospective study included diabetic and non diabetic patients registered at Asian Bariatric Pvt.Ltd. Diabetic and non-diabetic patients those who were willing to give written informed consent, were enrolled in the study. Patients were enrolled according to the inclusion and exclusion criteria. Every patient had all the right to refuse to give the consent. Blood collection of the patients was done and their lab investigation data were collected.. The small portion of stomach was cut and taken with the help of the surgeon. The stomach was stored in PBS at -80⁰C. From the stomach portion, DNA was isolated from 40 tissues. The sequencing of the DNA was carried out after DNA isolation.