EVALUATING THE ROLE OF SCFAs IN COMBINATION TO REVERSE THE DIABETIC CHARACTERISTICS

Abstract

Short chain fatty acid (SCFAs) are gut bacterial fermentation product. The most predominant SCFAs found in gut are Acetate, propionate and butyrate, present normally in the ratio of 60:25:15 respectively. In many studies, SCFAs are found to have anti-inflammatory effect. In the previous studies from our institute, microflora alteration was seen in diabetic group along with the SCFAs alteration which was restored by use of single SCFA. On that basis, we have hypothesized that HFD+HSD fed mice are treated with SCFAs combination for activation of FFARs and checked for reversal of inflammation, reversion to insulin sensitivity and restoration of gut flora. This research aimed to investigate the anti-diabetic characteristics of all the combined SCFAs group, to perform SCFA profile and to check for restoration of gut flora. The C57/BL6 mice were divided into three different groups: Normal control group (control diet), Diabetic or HSD+HFD group and treatment group with SCFAs combination (AP, AB and BP). After 60 days of induction and 60 days of treatment study, significant changes in different biochemical parameters such as body weight, OGTT, fasting glucose, SGOT, cholesterol and triglyceride level have been observed. Also, gene expression of liver and histopathological analysis of pancreas was done and considerable decrease in inflammation was found in combination treatment mice.