MHC II Gene Polymorphism in Malaria Patient

Abstract

Over the last 50 years, polymorphism in the MHC class II locus have been shown to influence individual’s susceptibility to infectious diseases. Impaired antigen presentation or unstable MHC class II molecules contribute to the insufficient CD4+ Tcell responses and increases susceptibility to infections. MHC II gene is highly polymorphic in nature. The highly conserved promoter region SXY module is there in MHC class II. The sequence variation in the promoter region of the MHC II varies the immune response in individuals. By doing promoter amplification and sequencing, the polymorphism observed in the patient samples at various sites. Transcription factor binding efficiency varies with the polymorphism. In the A9 sample BTEB, COE1 and NF-Y transcription factors bind additionally and in the A33 sample BTEB transcription factor also binds additionally and Elk1 doesn’t bind while comparing to the all positive and normal healthy samples which function in the B-cell differentiation. We got the high DRB expression with the low parasitic load in the samples which are having polymorphism in various sites. This may be due to transcription factor binding efficiency. So, this transcriptional regulation of MHC II gene may contribute to the optimal design, assessment of vaccines and further elucidation of the association between immunogenicity and protection.