Neuroprotective effect of potassium channel openers against hydrogen peroxide (H2O2) induced neuronal stress: IN-VITRO study

Abstract

Aim: This study was design to assess and establish in-vitro neuro protective role of potassium channel openers (KCOs) against hydrogen peroxide (H2O2) induced neuronal stress. Material and methods: Cell viability assay was done using MTT (3-(4, 5 dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide) method to determine IC50 value of H2O2. Based on 50 % Inhibitory Concentration (IC50) results of H2O2, neuroprotective effect of KCOs cromakalim, nicorandil , cinnarizine, and standard vitamin E was determined using U87 cells (human primary glioblastoma cell line). Results: Cromakalim, nicorandil and vitamin E significantly produce neuroprotective effects against H2O2 induced neuronal damage while cinnarizine did not produce significant activity. Conclusion: Results of our study demonstrated the neuroprotective role of potassium channel openers against H2O2 induced oxidative damage to neuronal cells.