In VitroEvaluation of Pramipexole Pamaote Salt

Abstract

The focus of the current study was to develop Pramipexole HCl (PRP) Nanoparticles to extend the biological time-period of PRP, based on the counter ion technique which was further observed for sustained release of the drug using in vitro dissolution method. In this regard, the molar ratio of a drug and counter ion i.e. Sodium Pamoate (Na-PAM) was quantified and then stable salt (1:1) was prepared which is called as microcrystals. Characterization of these microcrystals was carried out on the basis of solubility, Differential Scanning Calorimetry (DSC), Infrared spectroscopy (IR), melting point and Mass spectrometry (MS). To determine the concentration of drug and counter ion in salt, the simultaneous equation was developed using UV-VIS spectroscopic method. Development of the Nanoparticles from microcrystals with the help of high-pressure homogenizer (HPH) has been done where α-Tocopherol Polyethylene glycol 1000 succinate (TPGS) was used as a stabilizer. In the next step, the particle size of the prepared Nanoparticles was exploited through particle size analyzer and found in the acceptable range. In vitro dissolution study was performed by means of dialysis bag method at 6.8 pH. The prepared Nanoparticles were able to extend the drug release up to 48 hrs. It was observed that Nanoparticles of Pramipexole has shown a significant sustained effect during in vitro dissolution and it might be a good option to provide compliance by minimizing the demand of daily dose for patient.