Evaluation of Wound Healing Property of Murivenna in Streptozotocin Induced Diabetic Rats

Abstract

es collectively known as ‘wound healing’. Amongst all, proliferative phase of wound healing is found to be most important owing to fibroblast proliferation and migration, angiogenesis and granulation tissue formation. A synaptic protein called β-Neurexin, is expressed on the cells of vasculature and is found to be dynamic during angiogenesis. By interacting with an another synaptic protein called Neuroligin, it contributes to angiogenesis. Thus, β-Neurexin is an important protein involved in Angiogenesis and irreplaceably contributes to wound healing. Ki-67 is a cell proliferation marker, hence contributes to proliferative phase of wound healing process. Murivenna oil is an ayurvedic medicine that fastens wound healing. It has been seen that ingredients of Murivenna improves the wound healing by affecting the proliferative phase mainly. Thus in the present study, β-Neurexin and Ki-67 expression has been evaluated during wound healing process with respect to Murivenna treatment during diabetic condition. In in-vivo work, wound was created in control and diabetic wistar rats in which the treatment groups were topically treated with Murivenna twice a day for the experimental period. The pictures of the wound were taken every day to analyze the rate of wound closure, which was found to be faster in control and even in Diabetic groups upon treatment with Murivenna as compared to untreated groups. Histological alteration and skin regeneration post wounds upon treatment was assessed by HE and Masson’s trichrome stain. Histology showed that Murivenna treatment improves wound healing by enhancing epidermis regeneration by re-epithelization and keratin synthesis, adipocyte proliferation, proliferation and migration of fibroblasts and muscle regeneration in control non-diabetic rats leading to increased rate of wound closure. Epidermis regeneration and fibroblasts proliferation was also improved upon treatment of Murivenna in diabetic rats. IHC showed that expression of β-Neurexin and Ki-67 increased during wound healing. However, expression of β-Neurexin and Ki-67 were quite higher in untreated diabetic rats than in treated diabetic group which could be due to high blood glucose levels of diabetic treated group. Through in-silico molecular docking, an effort has been made to identify phytochemicals of Murivenna, which acts via β-Neurexin for improving wound healing process. Folic Acid, Isorhamnetin 4’-Glucoside, Quercetin 4’-Glucoside, Gamma Oryzanol , Pongamone D, Ponganone 4, Ponganone 5, Peonidin 3-glucoside, Robustone, Dihydroquercetin 3-glucoside, Erythrinin A, Erythrabyssin II, Laburnetin are the successfully docked phytochemicals in ascending orders with binding energy (ΔG) ranging from -10.3 kJ/mol to -9 kJ/mol. Most of the successfully docked phytochemicals belonged to Allium cepa and Pongamia pinnata.