IR @ Nirma University
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http://10.1.7.192:80/jspui/handle/123456789/8433| Title: | Cloning of Sar1a GTPases Homologue from Entamoeba Histolytica |
| Authors: | Patel, Yesha Memon, Shifa |
| Keywords: | Biochemistry Project Report Project Report 2019 Biochemistry Project Report 17MMB 17MMB024 17MBT 17MBT036 |
| Issue Date: | Apr-2019 |
| Publisher: | Institute of Science, Nirma University |
| Series/Report no.: | ;SDR00336 |
| Abstract: | Conclusion In this study, we successfully identified the Sar1a GTPase from E. histolytica genome database. The identified putative 573-bp of Sar1a GTPase sequence (EHI_031410A) was efficiently cloned into a pEhex-HA amoebic expression vector. The sequence identity and fidelity of cloned Sar1a GTPase (573-bp) was confirmed by automated Sanger sequencing. The confirmed pEhex-HA-Sar1a construct will be further used in sub-cellular localization and functional studies in E. histolytica trophozoite. |
| Description: | SDR00336 |
| URI: | http://10.1.7.192:80/jspui/handle/123456789/8433 |
| Appears in Collections: | Dissertation, BC |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| SDR00336.pdf | SDR00336 | 1.17 MB | Adobe PDF |  View/Open |
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