Please use this identifier to cite or link to this item: http://10.1.7.192:80/jspui/handle/123456789/9494
Title: To Assess if Carbon Nano - Dots are Maintained in Nano From In - Vitro and to address Their Cellular Safety Concern
Authors: Chatterjee, Shriya
Shree, Nidhi
Kumar, Shivani
Joshi, Surbhi
Keywords: Biochemistry
Project Report
Project Report 2020
18MBT
18MMB
18MBT005
18MBT19
18MBT033
18MMB027
Issue Date: May-2020
Publisher: Institute of Science, Nirma University
Series/Report no.: ;SDR00373
Abstract: Nanotechnology is described as the science and engineering which deals with design, characterization, production and application of particles having size ranges (approx.<100nm). Nanomaterial behave significantly different than the bulk material due to surface effect and quantum effect. Research with nanoparticles have shown that, some physicochemical characteristics like chemical reactivity, degree of agglomeration, shape, size, solubility, surface area and composition could have profound effect on the biological system. Carbon dots a type of inorganic NP, is in great attention due to its wide range of significant properties such as biocompatibility, high resistance and chemical inertness. We have used carbon nano dots that possess fluorescence property. Carbon dots are exposed to lymphocytes to check the biological outcome at genetic level (genotoxicity). Our approach is to assess that if carbon dots are maintained in nano-form in-vitro and to address their cellular safety concern. To fulfil our approach, we have performed Cytokinesis block micronucleus assay (CBMN), and comet assay in which lymphocytes are exposed with carbon dots. CBMN is used to measure DNA damage, cytostasis and cytotoxicity.Once divided cell were scored, micronuclei frequency and nuclear division index were determined. Taking EMS as positive control (150μl) and C-Dots as treatment group (100μl and 150μl) we scored Nuclear Division Index (NDI) to ascertain the cytostatic effect and proliferation rate of cells following the treatment. Frequency of genetic damage in terms of micronuclei, in cells treatd with C-Dots was significantly higher as compared to negative control. Comet assay is DNA damage quantification method, that is easy to perform and highly reproducible. We have performed this for assessing DNA fragmentation due to treatment of C-Dots, which would thus cause genetic damage. In our experiment it was observed, that C-dots were inducing genetic damage at similar extent to EMS (positive control) during in vitro studies. But, as the number of cells obtained during this assay were low and so were the number of comet. Therefore, the results remain inconclusive.
Description: SDR00373
URI: http://10.1.7.192:80/jspui/handle/123456789/9494
Appears in Collections:Dissertation, BC

Files in This Item:
File Description SizeFormat 
SDR00373.pdfSDR00373756.83 kBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.