Novel Bio-analytical Technique for Estimation of Gamma Oryzanol in Rat Plasma and Brain Homogenate Using HPLC

Abstract

Gamma oryzanol, a component of rice bran oil is used for its anticancer and antihyperlipidemic properties. Bioanalytical method for rat plasma and brain homogenate was developed by HPLC system with a PDA detector in which drug elution was performed using C-18 column (4.6 mm ×150 cm, 5 ) with 1% acetic acid in methanol: acetonitrile (65/35, v/v) as mobile phase at 1.2 ml/min flow rate and detected at 326 nm wavelength. Liquid liquid extraction method was chosen for extraction of oryzanol from plasma as well as brain homogenate as it provided highest recovery (95% in plasma, 85% in brain homogenate). Various extraction solvents for each body fluid were analysed, out of which highest recovery for plasma (95%), in acetone: IPA (1/1, v/v) and for brain homogenate (85%) in isopropyl alcohol (IPA) was observed,