Enzymatic Hydrolysis Of Cellulosic Wastes

Abstract

In order to produce the Bioethanol from agricultural residues such as Wheat straw, Rice straw and Jawar straw, it is necessary to decompose the straw into soluble sugars. Enzymatic hydrolysis is one of the method in common use, while pretreatment is the effective way to increase the hydrolysis rate. The optimal conditions of pretreatment using Boiling water, Acid and Alkali enzymatic hydrolysis of straws were determined. The evaluation of boiling water, alkali and acid treatment was done with concentration effect and time effect on lignin removal. The enzymatic hydrolysis of evaluated pretreated wheat straw, Rice straw and Jawar straw by Trichoderma Reesei enzyme system was studied. At favorable conditions, temperature 50ºC, pH 4 and 30 RPM at which enzyme gives maximum activity and rate of hydrolysis was proved. The pretreatment effect on enzymatic hydrolysis is studied. The kinetics of enzymatic hydrolysis was determined and maximum rate was obtained. Study proves that there is sugar inhibit the reaction and rate of hydrolysis. The results of SEM, XRD analysis showed that the structure and the surface of the straw were changed and crystallinity decreased, through pretreatment that is in favour of the following enzymatic hydrolysis.