IR @ Nirma University
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http://10.1.7.192:80/jspui/handle/123456789/4681| Title: | Screening, Isolation and Purification of Amylase from Germinating Seeds of Brassica Nigra and lipase from germinating seeds of Linum Usitastissimum |
| Authors: | Upadhyay, Dhwani Rank, Jalpa |
| Keywords: | Biotechnology Project Report Biotechnology Project Report 2014 12MBT 12MBT008 12MBT021 |
| Issue Date: | Apr-2014 |
| Publisher: | Institute of Science |
| Abstract: | Black mustard and Flaxseed were purchased from marked and germinated. Screening for amylase in black mustard was done by starch agar plate assay, starch – iodine tube assay and zymography. Presence of Lipase in flaxseed was confirmed after getting positive results on olive oil agar, tributyrin agar plates and zymography. Quantitative analysis of amylase was performed by DNSA method. Various types of chromatography was done ( Ion- Exchange chromatography with DEAE sephadex A-50 and Q sepharose, Gel filtration chromatography with sephadex G -75 and gel filtration high performance liquid chromatography) to purify the proteins. |
| URI: | http://hdl.handle.net/123456789/4681 |
| Appears in Collections: | Dissertation, BT |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| SDR00212.pdf | SDR00212 | 3.01 MB | Adobe PDF |  View/Open |
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