Biodegradation of Petroleum Oil Sludge by Bioaugmentation of Indigenously Isolated Microflora

Abstract

CONCLUSION • Screening of 40 bacterial isolates was done by investigating their sodium benzoate degradation efficiency, out of which 27 were fast growers. • 5 isolates had more than 80% sodium benzoate degradation efficiency and hence, those isolates (AWCO15, ASS Phn66, AWS ant33, AWDS21 and AWIS04 ) were further investigated for their growth and degradation capacity on poly aromatic hydrocarbons and crude oil. • A consortium of these 5 bacterial isolates were developed and it was observed that, the consortium showed the highest crude oil degradation efficiency i.e. 91.18 % • Identification of two isolates (AWCO 15 and ASS Phn66) from the consortium was done by genomic DNA isolation and 16S rRNA gene amplification and identified as Acinetobacter lwoffii and Burkholderia multivorans respectively. Identification of 3 isolates are still pending and are under progress.